13:00 – 15:00

SAFC Biosciences Workshop 
Integrated Strategies for Cell Line Selection
and Process Optimization
Abstract  
Registration closed!  
The workshop will take place in room S3 & S4.

15:30

Welcome

15:45

Invited Lecture
Delivery of biomolecules with non-viral vectors
Patrick Erbacher, Polyplus-Transfection SA, Illkirch/F

O-3.01

16:05

Invited Lecture
Circumventing the “pay now or pay later” dilemma: strategies for achieving process development with speed and long-term potential
Carole Heath, Amgen, Seattle, WA/USA

O-3.02

16:25

Balancing speed, throughput and cell culture yield: can we have it all?
T.S. Charlebois , M.W. Leonard, Wyeth BioPharma, Andover MA/USA

O-3.03

16:45

Development of a high yield Phase III cell culture process: efficient process optimization strategies for improving growth and productivity and maintaining product quality
K.M. Jamison , M. Arulgnanendran, D. Brooks, R.Shawley, M.Gawlitzek, Genentech, Inc., South San Francisco, CA/USA

O-3.04

17:05

VelociMab: mouse to manufacturing
J.P. Fandl , G. Chen, J. Hamilton, R. Babb, T. Daly, K.Bailey,
N. Papadopoulos, Regeneron Pharmaceuticals, Tarrytown, NY/USA

O-3.05

17:25

Coffee Break

17:55

Impact of stoichiometry on the productivity and quality of complex bioproducts: the case of retroviral vectors
A.S. Coroadinha , P.M. Alves, IBET/ITQB, Oeiras/P; M.J.T.Carrondo, IBET, Oeiras/P

O-3.06

18:15

Automated screening of HEK293 clones to search for high producers of monomeric proteins
P. Söhlemann , E. Casademunt, C. Glass, C. Wegmann, C.Schröder, Octagene GmbH, Martinsried/D

O-3.07

18:25

Clone evaluation and process development using the BioProcessors Corp SimCell robot
G. Wilson, Novo Nordisk, Copenhagen/DK

O-3.08

18:35

Recombinant human antibody therapeutics, supply strategies for early and clinical development from CHO cells
R. Field , L. Daramola, D. Hatton, J. Dempsey, Cambridge Antibody Technology/UK

O-3.09

18:45 – 21:00

Traders Reception
sponsored by all exhibiting companies

08:30

Keynote Lecture
Glycoproteins: folding, quality control, and secretion
Ari Helenius, ETH Zurich/CH

KN-1

09:15

Novel strategies for generating optimized mammalian cell systems for production of biopharmaceuticals
E. Becker, L. Florin, J. Fieder, S. Grammatikos, H.Hoffmann, H. Kaufmann , Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach (Riss)/D

O-1.01

09:35

Targeted gene knockdown effects on recombinant protein secretion in CHO cells
T. Borgschulte, SAFC Biosciences, St. Louis, MO/USA; F.Delegrange, EPF, Lausanne/CH; C.L. Bausch, SAFC Biosciences, St. Louis, MO/USA; D. Hacker, EPF, Lausanne/CH; D.W. Allison, M.V. Caple, SAFC Biosciences, St. Louis, MO/USA; F. Wurm, EPF, Lausanne/CH; K.J.Kayser , SAFC Biosciences, St.Louis, MO/USA

O-1.02

09:55

RNA silencing suppressors boost the production of proteins and viruses
T. de Haan , Phytovation BV, Leiden/NL; A. Engel, Roche Diagnostics GmbH, Penzberg/D; J. van der Velden, G.-J. van Holst, E. Arnoldussen, Phytovation BV, Leiden/NL

O-1.03

10:15

Synthetic positive feedback loops for controlled cell expansion
T. May, S. Bantner, D. Wirth , Helmholtz Centre for Infection Research, Braunschweig/D

O-1.04

10:35

Coffee Break

11:05

Fine control of heavy and light chain gene expression in CHO cells for efficient monoclonal antibody production
Y.S. Yang , M.G.S. Yap, Bioprocessing Technology Institute, Singapore/SGP

O-1.05

11:15

LMPC of single living cells opens a new field of applications on stem cell research
M. Stich , Y. Niyaz, PALM Microlaser Technologies GmbH, Bernried/D;
A. Buchstaller, University of Munich/D; R.Gangnus, R. Burgemeister, PALM Microlaser Technologies GmbH, Bernried/D

O-1.06

11:25

Designed protein storage organelles: a new approach for engineered protein expression in eukaryotic cells
P.B. Heifetz, M. Bastida, B. Llompart, ERA Biotech, Barcelona/E; I. Llop-Tous, CSIC-IRTA, Barcelona/E; P.Marzábal, K. O'Connor, J.M. Sly , ERA Biotech, Barcelona/E; S. Paz, M. Torrent, D. Ludevid, CSIC-IRTA, Barcelona/E

O-1.07

11:35

Keynote Lecture  
Systems biology: network of networks
Ruedi Aebersold, ETH Zurich/CH

KN-2

12:20

Lunch Break

13:20

16:20

Modifying cellular properties using microarrays
P. Jaluria, National Institute of Diabetes & Digestive & Kidney Diseases, Bethesda, MD/USA; M. Betenbaugh, K. Konstantopoulos, John Hopkins University, Baltimore, MD/USA; J. Shiloach , National Institute of Diabetes & Digestive & Kidney Diseases, Bethesda, MD/USA

O-4.01

16:40

Cell-to-cell communication for cell density-controlled bioprocesses
W. Weber , ETH Zurich/CH; M. Daoud-El Baba, Université de Lyon/F;
M. Fussenegger, ETH Zurich/CH

O-4.02

17:00

A predictive model for the GS-NS0 system under normal and hyperosmotic culture conditions
Y. Ho, J. Varley, A. Mantalaris , Imperial College London/UK

O-4.03

17:20

Monitoring and optimal control of the fluxome in recombinant BHK cultures
A.P. Teixeira, N. Carinhas, IBET/ITQB, Oeiras/P; C.Portugal, J.G. Crespo, FCT/UNL, Caparica/P; P.M.Alves, M.J.T.Carrondo, IBET/ITQB, Oeiras/P; R. Oliveira , FCT/UNL, Caparica/D

O-4.04

17:40

Differential expression profiling of industrially relevant CHO cell phenotypes using a proprietary CHO-specific microarray and proteomics technology platforms
M. Melville , M.S. Sinacore, D. Di Nino, K. McCarthy, K.Anderson,
K. Kopycinski, G.W. Lee, S. Max, Wyeth BioPharma, Andover, MA/USA;
P. Gammell, P. Doolan, P.Meleady, N. Barron, M. Leonard, M. Clynes, T. Charlebois, National Institute for Cellular Biotechnology, Dublin/IRL

O-4.05

18:00

Metabolite analysis in mammalian cells: how to generate reliable data sets?
J.B. Ritter , Y. Genzel, MPI for Dynamics of Complex Technical Systems, Magdeburg/D; U. Reichl, Otto-von-Guericke University and MPI for Dynamics of Complex Technical Systems, Magdeburg/D

O-4.06

18:10

Metabolome analysis in mammalian cells: development and application of a sampling technique
C. Wiendahl , Research Center Jülich GmbH/D; J.J.Brandner, U. Schygulla, Research Center Karlsruhe/D; S. Mazurek, H.Scheefers, SchBo Biotech AG, Giessen/D; S. Ritchie, Phenomenome Discoveries Inc., Saskatoon/CDN; T. Noll, University of Bielefeld/D; M. Oldiges, Research Center Jülich GmbH/D

O-4.07

18:20 – 19:05

Keynote Lecture
Lipid rafts in cell physiology and disease
Kai Simons, MPI of Molecular Cell Biology and Genetics, Dresden/D

KN-3

08:30

Novel scaffolds for tissue engineering - nano-structured surfaces promote selective cell attachment and cell differentiation
S. Asgari , M. Kürschner, Cinvention AG, Wiesbaden/D

O-6.01

08:50

Cryopreservation of adherent cells: strategies to improve cell viability and neuronal differentiation after thawing
R. Malpique, IBET/ITQB, Oeiras/P; F. Ehrhart, A.Katsen-Globa, Fraunhofer IBMT, St. Ingbert/D; M.J.T.Carrondo, P.M. Alves, IBET/ITQB, Oeiras/P; H. Zimmermann , Fraunhofer IBMT, St. Ingbert/D

O-6.02

09:00

Transcriptional profiling to identify markers for cell line engineering and selection
R. Deshpande , J. Wu, J. Cai, Amgen, Thousand Oaks, CA/USA

O-6.03

09:10

GlycoProfiling of cell lines used for production of biotherapeutics with optimised glycosylation
H. Baumeister , L. Stöckl, S.Goletz, Glycotope GmbH, Berlin/D

O-6.04

09:20

Recombinant bacterial vector for antigen delivery: from the proof of concept to the high throughput T-cell vaccine design
M. Derouazi , O. Epaulard, B. Toussaint , B. Polack, CNRS UMR 5525, Grenoble/F

O-6.05

09:40

Scale-down approach for animal-free polio vaccine production
W.A.M. Bakker , Y.E. Thomassen, L.A. Van der Pol, Netherlands Vaccine Institute, Bilthoven/NL

O-6.06

10:00

Invited Lecture
Scale-down models and miniturization for cell culture processes
Ashraf Amanullah, Genentech Inc., Oceanside, CA/USA

O-6.07

10:20

Unconventional experimental concepts enabling high speed high performance media development
M. Jordan , D. Voisard, N. Bencherif, A. Berthoud, L.Tercier, B. Kleuser,
G. Baer, Merck Serono SA, Fenil-sur-Corsier/CH

O-6.09

10:30

Poster Session 2 for posters with uneven numbers

12:40

Lunch Break

13:40 – 14:40

Poster Viewing

14:45

Excursion to Pillnitz and Königstein

09:00

Invited Lecture 
Gene modified hematopoietic stem cells for the treatment of primary immunodeficiency diseases   
Manuel Grez, Georg-Speyer-Haus, Frankfurt am Main/D; M.G. Ott, Medical School, Frankfurt am Main/D; S. Stein, Georg-Speyer-Haus, Frankfurt am Main/D; A. Schilz, EUFETS AG, Idar-Oberstein/D; U. Siler, University-Children's Hospital, Zurich/CH; K. Kühlcke, EUFETS AG, Idar-Oberstein/D; R. Seger, University-Children's Hospital, Zurich/CH

O-2.01

1091/
09:20

An avian cell line for production of highly attenuated viral vectors
I. Jordan , D. Horn, K. Höwing, V. Sandig, ProBioGen AG, Berlin/D

O-2.02

1205/
09:40

Human embryonic stem cell (hESC) bioprocess development: enhancing cardiac cell generation using hypoxia
S. Niebrügge , C.L. Bauwens, R. Peerani, P.W. Zandstra, University of Toronto/CDN

O-2.03

1217/
10:00

Controlling the embryonic stem cell microenvironment in culture: inherent variations associated with manual handling and opportunities for automation
C. Mason, R. Scott, J.-W. Wong, G.J. Lye, F.S. Veraitch , University College London/UK

O-2.04

10:20

Invited Lecture
From RNAi phenotypes to molecular function: a pipeline to validate and characterize hits from RNAi screens in mammalian tissue culture cells
R. Kittler, I. Poser, L. Pelletier, A. Heninger, M. Slabicki, M. Theis, A.A. Hyman, and Frank Buchholz , MPI of Molecular Cell Biology and Genetics, Dresden/D

O-2.05

10:40

Coffee Break

11:10

Keynote Lecture
Cell free protein synthesis
James Swartz, Stanford University, CA/USA

KN-4

1206/
11:55

Human cell lines for production of biopharmaceuticals
G. Schiedner, N. Gaitatzis, S. Hertel, C. Bialek, H.Kewes, C.Volpers,
G. Waschütza, CEVEC Pharmaceuticals GmbH, Köln/D

O-5.01

12:15

Invited Lecture
Directed glycosylation of therapeutic proteins in Yeast“
Stefan Wildt, GlycoFi, Inc., Lebanon, NH/USA

O-5.02

12:35

Lunch Break

13:35

Poster Viewing

14:35

Invited Lecture
Opportunities and challenges for biopharmaceuticals from plant cells
Rainer Fischer, Fraunhofer IME, Aachen/D

O-5.03

14:55

Invited Lecture
Recombinant expression in mosses: fast, flexible, sophisticated
Gilbert Gorr, greenovation Biotech GmbH, Freiburg/D

O-5.04

15:15

Coffee Break

15:45

Invited Lecture
Development of edible plant vaccines
Heike Mikschofsky , University Rostock/D; P. König, G. Keil, H. Schirrmeister, Federal Research Centre for Virus Diseases of Animals, Greifswald-Insel Riems/D; I. Broer, University Rostock  

O-5.05

16:05

Invited Lecture
Therapeutic proteins from transgenic cow milk
Carlos Melo, Bio Sidus S.A., Buenos Aires/RA

O-5.06

16:25

Keynote Lecture
Impressions from a nascent Biotech consultant and thoughts on a huge unmet need
W. Robert Arathoon, Biotechnology Consultant

KN-5

17:10 – 17:35

Closing

18:30

Social Event Hofkirche and Conference Dinner

Best Poster Award sponsored by